ESPEYB15 11 Obesity and Weight Regulation BAT: The story goes on (3 abstracts)
11.15 Three-Dimensional Adipose Tissue Imaging Reveals Regional Variation in Beige Fat Biogenesis and PRDM16-Dependent Sympathetic Neurite Density
Chi J , Wu Z , Choi CHJ , Nguyen L , Tegegne S , Ackerman SE , Crane A , Marchildon F , Tessier-Lavigne M & Cohen P
The Rockefeller University, New York, NY, USA
To read the full abstract: Cell Metab. 2018, 27, 226-236
Upon sustained cold exposure, white adipose tissue (WAT) can undergo substantial remodeling, characterized by the appearance of thermogenic brown-like or beige adipocytes. This “browning” of white fat depot also happens in humans and is associated with anti-obesity and anti-diabetic effects. This is why WAT “browning” is considered as a highly promising therapeutic target in obesity prevention and treatment. Interestingly, browning capacity differs upon WAT depot location and the heterogeneity of browning within one adipose depot was not well understood.
Tissue clearing has been used since the beginning of the last century to microscopically assess thicker tissue samples (1). Here, Chi et al. used this method to clear both visceral and subcutaneous adipose tissue of cold-exposed mice in order to image adipose browning in the complete fat depot by light sheet fluorescence microscopy. UCP1 positivity was detected only in subcutaneous fat and was stronger in the inguinal depot. Epididymal visceral fat was resistant to browning, as reported earlier, and showed less sympathetic nerve projections compared to subcutaneous fat.
These observations are critical when investigating brown adipogenesis in WAT with conventional methods – one should be aware of the heterogeneity of browning potential. It would be interesting to see whether this method could be transferred to human WAT browning.
1. Spalteholz, W. Über das Durchsichtmachen von menschlichen und tierischen Präparaten. (1911).
Volume 15
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ESPE Yearbook of Paediatric Endocrinology
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