ESPEYB15 11 Obesity and Weight Regulation BAT: The story goes on (3 abstracts)
11.16 Non-invasive Measurement of Brown Fat Metabolism Based on Optoacoustic Imaging of Hemoglobin Gradients
Reber J , Willershäuser M , Karlas A , Paul-Yuan K , Diot G , Franz D , Fromme T , Ovsepian SV , Bézière N , Dubikovskaya E , Karampinos DC , Holzapfel C , Hauner H , Klingenspor M & Ntziachristos V
Institute for Biological and Medical Imageing (IBMI), Helmholtz Zentrum München, Neuherberg, Germany
To read the full abstract: Cell Metab. 2018, 27, 689-701
The gold standard for the determination of BAT activity is the measurement of glucose uptake upon cold exposure using [18F]-FDG PET/CT. However, due to the use of ionizing radiation, this technique cannot be used repeatedly in longitudinal studies in humans. Alternatively, magnetic resonance imaging (MRI) and near-infrared fluorescence imaging (NIR) have been used to assess blood flow in BAT, but those methods do not accurately distinguish between WAT and BAT.
MSOT is an imaging technique that illuminates tissue with transient light. The light pulses are absorbed by the tissue, which then undergo thermo-plastic expansion that gives rise to detectable ultrasound waves. MSOT can scan tissues using light of different wavelengths, which excites different photo-absorbing molecules in the specimen (1). Here, the authors used light of spectral range 700-970 nm, which excites oxygenated hemoglobin. The system is limited by the low penetration depth of 5 cm, but provides high-resolution real-time images in situ and is able to detect BAT activation in mice and humans. Because MSOT does not require ionizing radiation or tracers, it is suitable for repeated assessments of brown fat activation, which is not possible by PET/CT.
1. Ntziachristos, V. & Razansky, D. Molecular Imaging by Means of Multispectral Optoacoustic Tomography (MSOT). Chem. Rev. 2010; 110, 2783–2794.
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