ESPEYB18 1. Pituitary and Neuroendocrinology Development/Ontogeny (6 abstracts)
1.2. Single nucleus multi-omics regulatory landscape of the murine pituitary
Ruf-Zamojski F , Zhang Z , Zamojski M , Smith GR , Mendelev N , Liu H , Nudelman G , Moriwaki M , Pincas H , Castanon RG , Nair VD , Seenarine N , Amper MAS , Zhou X , Ongaro L , Toufaily C , Schang G , Nery JR , Bartlett A , Aldridge A , Jain N , Childs GV , Troyanskaya OG , Ecker JR , Turgeon JL , Welt CK , Bernard DJ & Sealfon SC
Nat Commun. 2021 May 11;12(1):2677. doi: 10.1038/s41467-021-22859-w. PMID: 33976139
This work generated an integrated single nucleus multi-omics resource to elucidate the epigenetic mechanisms that regulate transcriptional networks in the murine pituitary. The authors identified epigenetically defined cell type composition, cell type-specific and sex-specific differences in transcriptional and epigenetic programs, an experimentally supported cis-regulatory domain, and epigenetic mechanisms contributing to cell type-specific and sex-specific regulon composition.
Based on this atlas, the authors focused on the Fshb gene, and applied co-accessibility analysis, identified a putative regulatory region, which corresponded to the human single nucleotide polymorphism rs11031006. The experimental validation that followed supports the value of co-accessibility analysis, available on the atlas web portal, for the identification of putative cis-regulatory domains for any genes of interest. This is the most comprehensive atlas related with murine pituitary until now, and provides valuable information, which supports researchers to conduct future study on pituitary development, and identify clinically important new putative regulatory domains.
Volume 18
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ESPE Yearbook of Paediatric Endocrinology
ISSN 1662-4009 (Online)
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