ESPEYB17 1. Pituitary and Neuroendocrinology Hypopituitarism: Disease Modeling and New Discoveries (3 abstracts)
1.5. Hypothalamic contribution to pituitary functions is recapitulated in vitro using 3D-cultured human iPS cells
Kasai T , Suga H , Sakakibara , Ozone C , Matsumoto R , Kano M , Mitsumoto K , Ogawa K , Kodani Y , Nagasaki H , Inoshita N , Sugiyama M , Onoue T , Tsunekawa T , Ito Y , Takagi H , Hagiwara D , Iwama S , Goto M , Banno R , Takahashi R & Arima H
To read the full abstract: Cell Rep. 2020 Jan 7;30(1):18–24.e5. doi: 10.1016/j.celrep.2019.12.009. PMID: 31914385.
The hypothalamic-pituitary axis influences many body organ systems and its dysfunction can be fatal. A proper in vitro model is important for understanding the disease mechanisms affecting pituitary function. Based on their previous anterior pituitary modelling with human embryonic stem cells (ESCs) (Ozone et al. Nat Commun, 2016), the authors investigated here whether hypothalamic-pituitary functional units can also be differentiated from human induced pluripotent stem cell (iPSC) lines. Indeed, using a long-term 3D culture, hypothalamic precursor-like cells were observed adjacent to the anterior pituitary tissue in the same cellular (hybrid) aggregates. They even showed that secretion of ACTH in cell aggregates was under the control of the hypothalamic CRH. These results provide fascinating possibilities for future human disease modeling of genes affecting the ontogeny and function of the hypothalamic-pituitary axis.
Volume 17
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ESPE Yearbook of Paediatric Endocrinology
ISSN 1662-4009 (Online)
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